A collection of reproducible methods for use in cancer detection. This work includes FISH-based methodologies used in the diagnosis of solid tumors, the molecular diagnosis of genetic abnormalities by DNA array technologies and methodologies directed at the detection of epigenetic events and at quantitative gene expression.

This book is a diverse collection of readily reproducible methods for use in cancer detection. Its highlights include FISH-based methodologies currently used in the diagnosis of solid tumors, the molecular diagnosis of genetic abnormalities by DNA array technologies - including sequence-specific oligonucleotide arrays and CGH arrays - and methodologies directed at the detection of epigenetic events and at quantitative gene expression. The authors apply these novel diagnostic procedures to a broad range of cases taken from cytology, solid tumor pathology, hematology, and rare cell detection, paying special attention to potential future developments and the practical problems of dealing with quality control and accuracy.

Prognostic and Predictive Factors
Michael Scott and Peter A. Hall
Assessment of Predictive Values of Tumor Markers
Joseph E. Roulston
Quality Assurance of Predictive Markers in Breast Cancer
Anthony Rhodes and Diana M. Barnes
Extraction of Nucleic Acid Templates
John M. S. Bartlett and Helen Speirs
Microdissection and Extraction of DNA From Archival Tissue
Joanne Edwards, James J. Going, and John M. S. Bartlett
Fluorescence In Situ Hybridization: Technical Overview
John M. S. Bartlett
HER2 FISH in Breast Cancer
John M. S. Bartlett and Amanda Forsyth
Fluorescence In Situ Hybridization for BCR-ABL
Mark W. Drummond, Elaine K. Allan, Andrew Pearce, and Tessa L. Holyoake
UroVysion
Multiprobe FISH in Urinary Cytology
Lukas Bubendorf and Bruno Grilli
Chromogenic In Situ Hybridization in Tumor Pathology
Jorma Isola and Minna Tanner
Comparative Genomic Hybridization and Fluorescence In Situ Hybridization in Chronic Lymphocytic Leukemia
Marie Jarosova
Molecular Characterization of Human Papillomaviruses by PCR and In Situ Hybridization
Suzanne D. Vernon and Elizabeth R. Unger
A Nested RT-PCR Assay to Detect BCR/abl
Linda M. Wasserman
TP53 Mutation Detection by SSCP and Sequencing
Jenni Hakkarainen, Judith A. Welsh, and Kirsi H. Vahakangas
PCR Diagnosis of T-Cell Lymphoma in Paraffin-Embedded Bone Marrow Biopsies
Jean Benhattar and Sandra Gebhard
Circulating DNA Analysis: Protocols and Clinical Applications Using Taqman Assays
Kwan-Chee Allen Chan and Yuk-Ming Dennis Lo
Microsatellite Instability: Theory and Methods
Gillian Gifford and Robert Brown
The Diagnostic and Prognostic Significance of the Methylation Status of Myf-3 in Lymphoproliferative Disorders
Jeremy M. E. Taylor, Peter H. Kay, and Dominic V. Spagnolo
Quantitative Analysis of PRAME for Detection of Minimal Residual Disease in Leukemia
Maiko Matsushita, Rie Yamazaki, and Yutaka Kawakami
Determination of Cyclin D1 Expression by Quantitative Real-Time, Reverse-Transcriptase Polymerase Chain Reaction
Karen E. Bijwaard and Jack H. Lichy
Detection of Telomerase hTERT Gene Expression and Its Splice Variants by RT-PCR
W. Nicol Keith and Stacey F. Hoare
Detection of Telomerase Enzyme Activity by TRAP Assay
W. Nicol Keith and Aileen J. Monaghan
Identification of TP53 Mutations in Human Cancers Using Oligonucleotide Microarrays
Wen-Hsiang Wen and Michael F. Press
Detection of K-ras Mutations by a Microelectronic DNA Chip
Evelyne Lopez-Crapez, Thierry Livache, Patrice Caillat, and Daniela Zsoldos
Microarray-Based CGH in Cancer
Ekaterina Pestova, Kim Wilber, and Walter King
Tissue Microarrays
Ronald Simon, Martina Mirlacher, Guido Sauter
Index