In the search for an effective treatment for Alzheimer's disease, APP is a unique model protein that illustrates the wide array of characterization techniques. This title presents biological techniques utilized in genetics, molecular biology, cell biology, and biochemistry to elucidate the structure and function of this transmembrane protein.

Practical in scope, it provides useful lists of protocols and routinely used antibodies for easy reference and paints a complete picture of the current understanding of APP as well as direction for future research.

Biochemical characterization of Amyloid Precursor Protein. Assays for the analysis of APP secretion and recycling. Strategies for crystallizing the N-terminal, growth factor domain of Amyloid Precursor Protein. Analysis of Amyloid Precursor Protein processing protease ss -secretase: tools for memapsin 2 (ss -secretase) inhibition studies. Assays for Amyloid Precursor Protein g-secretase activity. Cell-free reconstitution of ss -amyloid production and trafficking. Studying amyloid ?-protein assembly. Intracellular accumulation of Amyloid ss protein and mitochondrial dysfunction in Down's syndrome. A link between Alzheimer's disease ss -Amyloid and lipids: A technical approach. Regulation of Amyloid Precursor Protein processing by Lithium. Immunocytochemical analysis of Amyloid Precursor Protein and its derivatives. Pathological detection of Amyloid ss protein and Amyloid Precursor Protein in brain. Creating Amyloid Precursor Protein transgenic lines in mice. Generation of Amyloid Precursor Protein Knockout Mice.